FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26451
Sequences flagged as poor quality0
Sequence length91
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA3021.1417337718800802No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.926240973876224No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.7977014101546255No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.6653812710294507No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.5822086121507694No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.5179388302899701No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.4914748024649352No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.4423273222184417No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGGGGGGTGGGGG960.3629352387433367No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAA940.35537408793618386No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGGGGGGGGGGGG940.35537408793618386No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.30244603228611394No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT780.2948848814789611No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.2608597028467733No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAA640.24195682582889116No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGGGGGGTTGGGG640.24195682582889116No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAA580.2192733734074326No Hit
GTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAA570.2154927980038562No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAA560.21171222260027975No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.2041510717931269No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGGGGGGTGGCGG530.20037049638955048No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.20037049638955048No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA500.18902877017882122No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGGGGGGGTGGGG480.18146761937166836No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAA470.17768704396809193No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.16634531775736267No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG430.16256474235378623No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.15878416695020983No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAA420.15878416695020983No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA400.15122301614305697No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.14744244073948054No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAA390.14744244073948054No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.1398812899323277No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT350.13232013912517485No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.13232013912517485No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCGGGGGTGGCGG340.12853956372159844No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.124758988318022No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA320.12097841291444558No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA320.12097841291444558No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGGGGGGTTGCGG320.12097841291444558No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGTAGAGG310.11719783751086915No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG300.11341726210729274No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAA300.11341726210729274No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1096366867037163No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA290.1096366867037163No Hit
GGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1096366867037163No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA280.10585611130013987No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATCTGTCTCTTATACACATC280.10585611130013987No Hit
ATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATC280.10585611130013987No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA270.10207553589656344No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.10207553589656344No Hit
AGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAA270.10207553589656344No Hit
CAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAA270.10207553589656344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)