FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186
Sequences flagged as poor quality0
Sequence length91
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.6404391582799633No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.5032021957913998No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT90.41171088746569073No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.41171088746569073No Hit
ATTCTATTCCATTCCATTGCAATCGAGTTGAATCCATTGCATTCCATTCC80.36596523330283626No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.32021957913998167No Hit
ATTCCATTCCATTCCATTCTATTCCATTCCATTGCAATCGAGTTGAATCC60.2744739249771272No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2744739249771272No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2744739249771272No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAA50.22872827081427266No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA50.22872827081427266No Hit
ATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAA50.22872827081427266No Hit
GAATGGAATGGAATGGAATGCAATGGATTCAACTCGATTGCAATGGAATG50.22872827081427266No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.22872827081427266No Hit
ATTCCATTCCATTCGATTCCGGAAGATTCCATTCCATTGCATTCCATTCC50.22872827081427266No Hit
GAATAGAATCAATCCGAATGTAATGGAATGGAATGCAACGGAATGGAATG40.18298261665141813No Hit
ATTCCACTCCATTCCATTGCACTCGGGTTGATTCCATTCCATTCCATTCC40.18298261665141813No Hit
GAATTAACCCGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAACG40.18298261665141813No Hit
ATCCATTCCATTGCATTCCATTCCATTCCATTCCCCTGCAGTCGGGTTGA40.18298261665141813No Hit
CCATTACATTCCATTCCATTCGAGTTGTTCCATTCCATTCCATTCCATTC40.18298261665141813No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18298261665141813No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1372369624885636No Hit
ATTCTATTCCGTAGCATTCCATTCCATTCCATACAATTCCATTCCATTCC30.1372369624885636No Hit
GATGTGTATAAGAGACAGGTATCAACGCAGAGTACGGGAAAAAAAAAAAA30.1372369624885636No Hit
ATTCTATTCCGTACCATTCCATTACATTCCATACAATTCCATTCCATTCC30.1372369624885636No Hit
GTACAGGGGAATGGAATGGAATGGAATGCAATGGAATGGAATCATCCGTA30.1372369624885636No Hit
AAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACCTGTCTCT30.1372369624885636No Hit
GAGTGGAATGGAATGGAATGGAATTGTATGGAATGTAATGGAATGGTACG30.1372369624885636No Hit
GAATGGAATCGAATGGAATGGAATGGAATGGAATGGAATCAACCCGAGTG30.1372369624885636No Hit
GAATGGAATGGAATGCAATGGAATGGAATCATCCGTAATGGAATGGAAAG30.1372369624885636No Hit
ATTCCATTCCATTCCATTCCATTCCATTCGATTCCATTCCATTCCAATTC30.1372369624885636No Hit
GAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAGCAAAAAAA30.1372369624885636No Hit
GAATTGGAATGGAATGGAATCGAATGGAATGGAATGGAATGGAATGGAAT30.1372369624885636No Hit
GAATGGAATGGAATTAACCCGAATAGAATGGAATGCAATGTAATGGAACG30.1372369624885636No Hit
ATTATTGATAACCTTGTTTTGCACAAAAAAAAAAAAAAAAAAAAAAAAAA30.1372369624885636No Hit
ATTCCATTCCACTCGGGTTGATTCCAATGTATTCCTTTCCATTCCATTCC30.1372369624885636No Hit
ATTCCATTCCATTCCACTCCATTCCATTGCACTCGGGTTTATTCCATTCC30.1372369624885636No Hit
TATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAA30.1372369624885636No Hit
GGTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTA30.1372369624885636No Hit
GTATCAACGCAGAGTACGGGGAATGGAATGGAATGGAATGCAATGGAATG30.1372369624885636No Hit
ATTCCATTCCATTCCATTCCATTCCTTTCCGGAAAATTCCATTCCATTGC30.1372369624885636No Hit
GAATGGAATGGAATGCAATGGAATGGAATCATCCGGAATGGAATGGAATG30.1372369624885636No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1372369624885636No Hit
GAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATCAACCCGACTG30.1372369624885636No Hit
TTTCCATTCCATTCCATTCCATTGCAATCGAGTTGATTCCATTGCTTTGC30.1372369624885636No Hit
CTCCATTACATTCCATTCCATTCGAGTTGTTCCATTCCATTCCATTCCAT30.1372369624885636No Hit
ATTGATAACCTTGTTTTGCACTAAAAGCAAAAAAAAAAAAAAAAAAAAAA30.1372369624885636No Hit
ATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAA30.1372369624885636No Hit
GAATGGAATGGAACGGAATGGAACGGAATGGAATGGAATGGAATGGAATG30.1372369624885636No Hit
CCCGAGTACAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGA30.1372369624885636No Hit
GAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAA30.1372369624885636No Hit
GAATGGAAAGGAATGGAATGGAATGCAATGGAATGGAATGGAATGCAATG30.1372369624885636No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTCTGTCTCT30.1372369624885636No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1372369624885636No Hit
GGAATGGAATCATCCGGAATGGAATGGAATGGAATGGAATGCAAAGCAAT30.1372369624885636No Hit
ATTCCATTCCATTCCATTCCACTCCATTCCATTGCACTCGGGTTTATTCC30.1372369624885636No Hit
GTACAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGCA30.1372369624885636No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1372369624885636No Hit
ATTCCATTCCATTGCAATCGAGTTGAATCCATTGCATTCCATTCCATTCC30.1372369624885636No Hit
ATTCCATTCCATTAGAATGCTATCGAGTTGATTCCATTCCATTCCATTCC30.1372369624885636No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1372369624885636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAC300.00608929228.33333272-73
Produced by FastQC (version 0.11.5)