FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881864
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59860.31808887358491367No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37760.200652119388011No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20350.10813746370619769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28850.044.6410261
TGGTATC29800.042.641152
CCACGCG507.030201E-427.50227421
TACGTAG400.00787427727.5000813
GTATCAA106000.026.0133291
CGATCAT858.8002525E-725.88173934
GGTATCA55650.025.8625071
CACGATC801.6092345E-524.0619332
ATATACG39750.024.0101872
GATATAC39750.023.9537261
CGCATTG39150.023.812337
TATACGC40750.023.415383
ATACGCA41300.023.3698754
CCCCGAA3350.022.98514212
GCGACGT600.002035660722.91734125
CCGAATT3600.022.91551614
TACGCAT42400.022.8284345
ACGCATT42700.022.7330516
AGAGTAC48950.022.52815611
GCCCCGA3450.022.31890511
Produced by FastQC (version 0.11.5)