FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414726
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA153401.084308905045924No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA82220.581172608688891No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54560.38565771746613836No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.38339579536956275No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49120.34720504182435324No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40890.2890312329030498No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT37290.26358460931657435No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.19339433925721305No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA26810.18950666065372376No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA23780.16808908580177362No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA20650.14596466029464364No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.1378358777600751No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.1345136796807297No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.1111876080597939No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA15590.1101980171425421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25200.063.2604641
TGGTATC26050.061.670612
TATCACG356.6111006E-660.715022
AGCGTCA1650.059.2431373
GCGTCAG1700.057.5006944
GCATCGT1600.055.785878
CATCGTC1650.054.0953879
GCAGCGT1900.053.6981431
TAGGCAT1800.051.9487425
ATATACG71600.048.1989062
GATATAC70800.047.0143171
CAGCGTC2100.046.5481762
ATACGCA76150.045.4306224
TACGCAT76950.044.7405475
TATACGC78000.044.4620743
ACGCATT79150.043.928126
CGCATTG78500.043.8029867
GCATTGT77800.043.6507878
TACTAGG2050.043.537112
CGTCAGA2350.043.407855
Produced by FastQC (version 0.11.5)