FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237829
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA35461.4909872219115414No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30901.2992528245083652No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27471.1550315562862392No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24621.035197557909254No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23320.9805364358425592No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA23130.9725475026174268No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22640.9519444642999803No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.900226633421492No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19270.8102460170963172No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA16790.7059694149998529No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.6008518725639009No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT12930.5436679294787431No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAA12890.5419860487997679No Hit
GTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAA12370.5201215999730899No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAA12040.5062460843715443No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.4835406952053787No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAA10910.45873295519049406No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA10180.4280386327991961No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.37884362293917057No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAA8810.3704342195442944No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTGGGGG8020.33721707613453367No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTGGCGG7520.31619356764734324No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.31577309747759946No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.31367074662888045No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAA7330.3082046344222109No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.3052613432340043No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAA7210.3031589923852852No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAA7190.3023180520457976No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.29096535746271485No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6880.28928347678373956No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAA5340.2245310706431932No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGGGGGGG5280.22200824962473037No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.20645085334420948No Hit
AGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAA4720.19846192011907715No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAA4700.19762097977958953No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.1963595692703581No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAA4360.18332499400830007No Hit
CAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAA4290.18038170282009344No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4270.17954076248060583No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.17197229942521727No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCCTGTCTCTTATAC4040.16986994857649823No Hit
GGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.16902900823701064No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3920.16482430653957256No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.16398336620008494No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3820.16061960484213447No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3820.16061960484213447No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.15977866450264686No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA3720.15641490314469642No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3710.15599443297495258No Hit
GCCCAATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATAC3650.15347161195648976No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA3600.15136926110777071No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAA3600.15136926110777071No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATCTGTCTCTTATACACATC3530.14842596991956405No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.14422126822212597No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.14253938754315076No Hit
ATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATC3390.14253938754315076No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.14211891737340696No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3310.1391756261852003No Hit
AGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAA3260.13707327533648125No Hit
GGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.13160716312981174No Hit
CTGTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAA3110.13076622279032415No Hit
CATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.12992528245083654No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.12866387194160508No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA2910.12235681939544799No Hit
CCATAAGGTCATGTATTGGGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.11983399837698515No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA2770.11647023701903468No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.11562929667954706No Hit
CCCATAAGGTCATGTATTGGGCATAAAAAAAAAAAAAAAAAAAAAAAAAA2680.11268600549134043No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2590.10890177396364616No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAA2580.10848130379390235No Hit
GGTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.10764036345441473No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.10721989328467092No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGGGTGGCGG2550.10721989328467092No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAA2520.10595848277543948No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTTGGGG2500.10511754243595188No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAA2470.10385613192672045No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2460.10343566175697666No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.10343566175697666No Hit
GTCCCATAAGGTCATGTATTGGGCATAAAAAAAAAAAAAAAAAAAAAAAA2380.10007190039902619No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.10007190039902619No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGGC259.130255E-568.010933
GATATAC12350.067.116061
ATATACG12800.066.416932
AGCGTCA401.9480285E-763.7602543
GACTTAT200.00321828663.7602541
TTCATGT200.00321828663.7602541
ATCATGT200.00321828663.7602541
TGGTATC7900.062.9531632
GTGGTAT7900.061.3389781
CATATAC700.060.7240451
ACATGTA356.588438E-660.7240452
TATACGC14000.060.420433
ATACGCA14350.059.539194
TACGCAT14550.058.428645
ACGCATT14600.057.6583826
GCATTGT14850.057.2482648
CGCATTG14800.057.1544577
CTATACA454.3974978E-756.675781
GCATCCC302.2497958E-456.675789
CAGCGTC552.82962E-854.099612
Produced by FastQC (version 0.11.5)