FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237829
Sequences flagged as poor quality0
Sequence length61
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227619.570321533538802No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990.5882377674715867No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.3090455747616985No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAA7180.3018975818760538No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5600.23546329505653218No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5230.21990589877601135No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.21738307775754848No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.21570119707857324No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.19257533774266383No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.18584781502676292No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4240.1782793519713744No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.1728132397647049No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.15893772416315924No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCCTGTCTCTTATACACATCTGACGC3070.12908434211134892No Hit
GCCCAATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATCTGACGC3060.12866387194160508No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATCTGTCTCTTATACACATCTGACGCTGCCG3010.12656152109288607No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.12530011058365464No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.11857258786775374No Hit
ATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATCTGACGCTGCCG2710.11394741600057184No Hit
GTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.11310647566108423No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.10595848277543948No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.10385613192672045No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAA2440.10259472141748904No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)