FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62740
Sequences flagged as poor quality0
Sequence length91
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA7641.2177239400701307No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTGGCGG4910.7825948358304113No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTGGGGG4720.752311125278929No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.6518967166082243No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.6471150781000956No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.6439273190946764No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.5482945489321007No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.5116353203697801No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA3170.5052598023589417No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGGGGGGG2860.4558495377749442No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.42237806821804275No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.39528211667197966No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.3665922856232069No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.2868983104877271No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGGGTGGCGG1690.26936563595792157No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAA1680.267771756455212No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAA1650.2629901179470832No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTTGGGG1600.2550207204335352No Hit
GTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAA1570.25023908192540645No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1560.24864520242269686No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA1510.2406758049091489No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAA1500.23908192540643924No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAA1480.23589416640102007No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGGGGCGG1480.23589416640102007No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGTTGGCGG1350.21517373286579536No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAA1310.20879821485495698No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1270.2024226968441186No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.1944532993305706No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.18170226330889383No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTTGCGG1120.17851450430347465No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAA1020.1625757092763787No Hit
GGCTAGGGGCATCCCAGGGAGGGTTTTTGTATGAGCCTGTGTCACAGTGT940.14982467325470195No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGGTGGGG940.14982467325470195No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAA940.14982467325470195No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA930.14823079375199236No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGTTGGGGG930.14823079375199236No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTGGGGGGGG910.14504303474657315No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.14344915524386356No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.14185527574115397No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.14026139623844436No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGATGGCGG810.1291042397194772No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTGGGGTGGCGG800.1275103602167676No Hit
GTCCTAACACTGTGACACAGGCTCATACAAAAACCCTCCCTGGGATGCCC790.12591648071405803No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG780.12432260121134843No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAA760.12113484220592924No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGGATGGCGG730.11635320369780046No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAA720.11475932419509084No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG680.10838380618425247No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGTGTGGCGG670.10678992668154287No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGGGTGGCGGGGG660.10519604717883328No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG650.1036021676761237No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10200828817341409No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAA630.1004144086707045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACA357.767085E-1085.063052
CTTTACA150.001021977385.063041
TATGATG150.001021977385.063045
GACATAT150.001021977385.063041
ATATGAT150.001021977385.063044
CTTAACA150.001021977385.063041
CATATGA150.001021977385.063043
CATATAC456.548362E-1175.6115951
ACATATG200.003199853763.797292
AACACAT200.003199853763.797294
GATATAC1000.063.7972871
TGATGCG200.003209997263.7464147
ATATACG1150.062.872692
TACGCAT1250.057.8428735
ATACGCA1250.057.8428734
CTATACA302.2305652E-456.7086941
TATACGC1300.055.618153
ACGCATT1300.055.618156
CGCATTG1300.055.5737957
GCATTGT1300.055.5737958
Produced by FastQC (version 0.11.5)