FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62740
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1629225.967484858144722No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.2709595154606312No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.18648390181702262No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAA1030.1641695887790883No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.15620019126554033No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.14185527574115397No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.11475932419509084No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11157156518967166No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAAT202.584526E-455.0430951
ATGGCTG150.00575856454.99920335
TAATGGC150.00575856454.99920333
TCAATAC202.5947473E-454.9992033
AATGGCT150.00575856454.99920334
AATACTA202.5947473E-454.9992035
GTGGTAT1500.047.704021
TGGTATC1550.046.1283652
TTCAATA257.800159E-443.999362
GAATGCT257.800159E-443.9993632
AGAATGC257.800159E-443.9993631
CAATACT257.800159E-443.999364
TGCTCAC257.800159E-443.9993635
ATACTAC257.800159E-443.999366
TACCAAC257.800159E-443.9993610
CTACCAA257.800159E-443.999369
TACTACC257.800159E-443.999367
ACTACCA257.800159E-443.999368
TTGAGGC358.575013E-539.28514515
TGTGTAT358.575013E-539.2851453