FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297729
Sequences flagged as poor quality0
Sequence length61
%GC32

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22610.7594154415592703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14320.48097430885133796No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.39163131572671794No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC8680.2915402933540233No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA7950.26702135163185314No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA7100.23847189894165502No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA6270.21059419807946153No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.19111339506732633No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA5270.1770066066792284No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT5080.17062496431318414No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG5020.16860970882917015No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.16525094968914683No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGT4830.16222806646312588No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAA4120.13838087656896036No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT3910.13132748237491143No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT3890.13065573054690674No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT3840.1289763509768951No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCA3810.12796872323488812No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA3470.11654894215880884No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTATA3300.11083905162076922No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTT3130.1051291610827296No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG3050.10244215377071095No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA3040.10210627785670862No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA3030.1017704019427063No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAA2990.10042689828669697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCC150.00577885955.00134325
TCTATCT150.00577885955.00134355
CGTGCGC251.1863865E-554.99210710
GCGCGAT251.1863865E-554.99210713
TGCGCGA251.1863865E-554.99210712
CTACGTG251.1863865E-554.9921077
ACTACGT251.1863865E-554.9921076
GTGCGCG251.1863865E-554.99210711
GTCTACC150.005782701554.99210415
TAAGCAT202.6123266E-454.9921045
CAGTGGT850.048.5713921
GTGGTAT15650.046.7816471
CCCTTGA601.8189894E-1145.8729821
GGCTACT303.477524E-545.8498572
ACGTGCG303.487896E-545.8267569
TACGTGC303.487896E-545.8267568
TGGTATC16250.044.693032
AGTGGTA1000.044.0158652
GCTACTA358.6424356E-539.2932743
CGATATG358.659565E-539.28007516
Produced by FastQC (version 0.11.5)