FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40763
Sequences flagged as poor quality0
Sequence length61
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24365.976007653999951No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.3974192282216716No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.21342884478571253No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.15209871697372618No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10548781983661655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1650.040.14421
CCTACTT558.435345E-734.9968823
TGGTATC1850.034.2281382
CTACTTG501.7785922E-532.99705524
AGGGGTT1350.032.58968412
GAGGGGT1350.032.58968411
CTTTCCT601.665232E-632.0804719
TTTCCTA601.665232E-632.0804720
TCCTACT601.665232E-632.0804722
CGAGGGG1400.031.42576810
TTGACCT350.004055588531.42576828
CTTGACC350.004055588531.42576827
ACTTGAC453.7342202E-430.55282826
TACTTGA453.7342202E-430.55282825
CAGAGTA1201.8189894E-1229.7890110
AGAGTAC1201.8189894E-1229.7890111
ATGGGAC653.1091167E-629.61274313
ACTTTCC653.1091167E-629.61274318
GACTTTC653.1091167E-629.61274317
ACTTTTT1052.055458E-1028.9133821
Produced by FastQC (version 0.11.5)