FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830966
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA55590.6689804396329092No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.4232423468589569No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA31080.3740225231838607No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA27450.3303384253988731No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27190.32720953685229No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.30759381250255724No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.23045467564256542No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT16310.1962775853645035No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA14350.1726905793979537No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA13940.16775656284372648No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.1294878490816712No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA10310.12407246505873885No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.11251964580981652No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA9260.11143656900523007No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8450.10168887776395184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13500.060.4681131
TGGTATC15150.053.8597832
GCGTCAG2250.047.2208184
GTATCAA72800.045.0279271
CCGTCAC409.3110016E-442.506419
AGCGTCA2550.041.665433
GGTATCA32700.040.9563981
GCAGCGT2600.040.881391
TATCAAC82450.040.0503582
CCTTGAT4500.038.721072
TACTCCG709.2470145E-636.427495
ATCAACG90750.036.293693
TCAACGC92050.035.781124
CAACGCA93700.035.241755
AACGCAG99250.033.3607036
CCCTTGA3850.032.0255241
ATATACG45250.030.7117942
GATATAC44200.030.58891
CGCATTG45600.030.3865267
CAGCGTC3550.029.9286862
Produced by FastQC (version 0.11.5)