FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830966
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28730.3457421843974362No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21840.26282663791298316No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12090.1454933174161157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19700.045.549431
TGGTATC20300.043.6344872
TACGTTC300.001925740936.665028
GTATCAA95600.028.475411
TTAAACG1600.027.50042322
TACGGTA400.00787824727.49545716
GGTATCA45100.026.487731
TATCAAC104600.025.7992692
AGAGTAC50100.025.68747111
CAGAGTA52250.024.94624910
TTAGACG1052.0991865E-723.5689533
ATCAACG114600.023.4181333
TCAACGC115150.023.161614
CGGGTAC952.3168159E-623.15546217
GTGCTAT1202.9687726E-822.9377251
GTCCACG600.002035589222.9156388
GTTCGGT600.002037014322.9128816
CAACGCA116600.022.897165
AACGCAG119850.022.390966
ACGGGAT12800.022.3400616
Produced by FastQC (version 0.11.5)