FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135602
Sequences flagged as poor quality0
Sequence length91
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA24491.806020560168729No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18911.3945222046872465No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13861.022108818454005No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA13210.9741744222061621No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.883467795460244No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA11300.8333210424625006No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA10700.7890739074644916No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.7086916122181088No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.5906992522234186No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA7760.5722629459742482No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT7070.521378740726538No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.513266765976903No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA5620.4144481644813498No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.38568752673264406No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.38200026548281No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.31046739723602895No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.29055618648692494No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.2868689252370909No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.27949440273742276No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA3700.2728573324877214No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.26843261898792053No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.24409669473901566No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA3290.242621790239082No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG2850.21017389124054217No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2800.20648662999070813No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.19468739399123908No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA2590.191000132741405No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.18805032374153774No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA2550.18805032374153774No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.18362561024173685No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA2480.18288815799177002No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.1821507057418032No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA2450.1806758012418696No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.1769885399920355No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.1762510877420687No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA2380.1755136354921019No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2280.16813911299243373No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA2260.1666642084925001No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGG2160.15928968599283197No Hit
GTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.15265261574313063No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAA2040.15044025899323019No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.14749044999336294No Hit
GAGTACGGGATACCAGCTTATTCAATTAAAAAAAAAAAAAAAAAAAAAAA1960.14454064099349567No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC1950.14380318874352888No Hit
GATATACGCATTGTGGCAGTACATCAAGCGAAAAAAAAAAAAAAAAAAAA1930.14232828424359523No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTG1910.14085337974366158No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAA1900.14011592749369478No Hit
GTGGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACAT1870.13790357074379433No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.13716611849382754No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA1850.1364286662438607No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.13495376174392706No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.13421630949396027No Hit
ATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAA1780.131266500494093No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAA1750.12905414374419255No Hit
GAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.12831669149422575No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTG1700.12536688249435848No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAATTAAAAAAAAAAAA1680.12389197799442485No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC1600.11799235999469034No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1600.11799235999469034No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1570.1157800032447899No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC1520.11209274199495582No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA1490.10988038524505539No Hit
GTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.10988038524505539No Hit
GTGGTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTT1470.10840548074512175No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC1450.10693057624518812No Hit
TTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.10324331499535407No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCT1370.1010309582454536No Hit
CAGTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTT1360.10029350599548678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5850.068.315491
GAATCAA200.003212707163.7732351
CAGTGGT401.9389881E-763.7732351
CCCTTGA652.7284841E-1158.8676031
CGACATT302.2481687E-456.666423
GACATTT302.2481687E-456.666424
TGGTATC7700.054.6426242
CATGACC250.007781652750.999784
AGTGGTA509.0813046E-750.999782
AGGCGTA250.007781652750.999783
TGACCTT250.007781652750.999786
GACCTTA250.007781652750.999787
CCACTTA354.8031934E-448.5891341
CCTTGAT952.3646862E-1144.7366452
GTATCAA30300.043.49771
AATAAGC1500.042.4998172
GGTATCA14250.041.7696081
AGCTGGT1700.039.999836
GCTGGTA1600.039.8435757
TATCAAC33850.039.0470962
Produced by FastQC (version 0.11.5)