FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135602
Sequences flagged as poor quality0
Sequence length61
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10260.7566260084659518No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8640.6371587439713279No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA7030.5184289317266707No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.3407029394846684No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.31562956298579664No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAA2580.19026268049143819No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA2280.16813911299243373No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG2150.15855223374286515No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.15339006799309746No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA2070.15265261574313063No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.11872981224465716No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTGTCTCTTATACA1600.11799235999469034No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAATTAAAAAAAAAAAAAAAAAAAAAAA1500.11061783749502219No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAA1450.10693057624518812No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGA1430.1054556717452545No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.10471821949528767No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACA1420.10471821949528767No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAGC150.00577357454.9977933
ACATCAC150.00577357454.9977944
TACTGGC150.00577357454.9977915
ACTGGCC150.00577357454.9977916
TTAAACG251.1822023E-554.9977922
ACCAGAA150.00577357454.9977950
GCTTAAA305.3944495E-754.9977920
CGGGAGA202.6059095E-454.99778717
ACGGGTC202.6059095E-454.99778716
ACTGCTT407.086419E-848.12306217
TGCTTAA407.086419E-848.12306219
GTGGTAT7250.045.9624941
CAGTGGT601.8189894E-1145.899191
ACCACTG553.8016879E-1044.99818814
CACTGCT553.8016879E-1044.99818816
CAGCCAA257.8334624E-443.9982317
GCAGCCA257.8334624E-443.9982316
CTGCTTA451.7891944E-742.7760618
TGGTATC8450.040.353412
AGTGGTA709.276846E-1139.2841342
Produced by FastQC (version 0.11.5)