FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3777
Sequences flagged as poor quality0
Sequence length91
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA852.2504633306857293No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411.0855176065660577No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA310.8207572147206778No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.6883770187979878No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT210.5559968228752978No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.5030447445062218No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAA160.4236166269526079No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA150.3971405877680699No Hit
ATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAA150.3971405877680699No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3971405877680699No Hit
ATTGATAACCTTGTTTTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3441885093989939No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATCTCAG120.3177124702144559No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3177124702144559No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3177124702144559No Hit
TTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAA110.2912364310299179No Hit
AAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACCTGTCTCT110.2912364310299179No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA110.2912364310299179No Hit
GGTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTA100.2647603918453799No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA100.2647603918453799No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTCTGTCTCT100.2647603918453799No Hit
TGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.23828435266084197No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.21180831347630394No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT80.21180831347630394No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.21180831347630394No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.21180831347630394No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.21180831347630394No Hit
TTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.21180831347630394No Hit
GCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAA80.21180831347630394No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACAAA80.21180831347630394No Hit
ACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGG70.18533227429176596No Hit
GAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAA70.18533227429176596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18533227429176596No Hit
ATTGATAACCTTGTTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.15885623510722796No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACATCTCAGGAT60.15885623510722796No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAG60.15885623510722796No Hit
ATTATTGATAACCTTGTTTTGCACAAAAAAAAAAAAAAAAAAAAAAAAAA60.15885623510722796No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.15885623510722796No Hit
ATTGATAACCTTGTTTTGCACTAAAAGCAAAAAAAAAAAAAAAAAAAAAA60.15885623510722796No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.15885623510722796No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.15885623510722796No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG60.15885623510722796No Hit
ACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13238019592268996No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.13238019592268996No Hit
GTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13238019592268996No Hit
GCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAA50.13238019592268996No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13238019592268996No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTT50.13238019592268996No Hit
ATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAA50.13238019592268996No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGGGGATGGCGG50.13238019592268996No Hit
GTGCAAAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACTGT40.10590415673815197No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10590415673815197No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGGGGGTGGCGGGGT40.10590415673815197No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10590415673815197No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10590415673815197No Hit
GGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAA40.10590415673815197No Hit
AAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACCCTGTCTC40.10590415673815197No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10590415673815197No Hit
TTTTAGTGCAAAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGAT40.10590415673815197No Hit
GGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAA40.10590415673815197No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGGTTTTTTTTTT40.10590415673815197No Hit
CAGGTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTT40.10590415673815197No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.10590415673815197No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10590415673815197No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10590415673815197No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10590415673815197No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA40.10590415673815197No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA40.10590415673815197No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAA40.10590415673815197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA605.456968E-1056.6666641
TCGAGGG250.007207432851.09
CTTTTTT250.007207432851.01
TATCAAC753.954483E-945.3333362
ATTGATA408.179359E-442.51
TTGATAA450.001460535837.777782
ATCAACG901.9845174E-837.777783
AACGCAG953.198329E-835.7894746
TCAACGC953.198329E-835.7894744
CAACGCA953.198329E-835.7894745
GTCGAGG601.3130048E-435.4166648
GATAACC500.002450928834.04
AACCTTG500.002450928834.07
TGATAAC500.002450928834.03
ATAACCT500.002450928834.05
TAACCTT500.002450928834.06
GTAAAAA250.00261686234.062-63
AGTCGAG652.1028721E-432.6923077
TTGCACT550.003911340630.909099
CAGAGTC703.250087E-430.3571414
Produced by FastQC (version 0.11.5)