FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754725
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG376120.6535846630377646No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180050.3128733345207634No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103650.18011286377715702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT154400.046.134661
TGGTATC158350.044.4009862
GGTATCA311500.024.9526671
GTATCAA565050.024.6962741
AGAGTAC224700.023.6923811
CAGAGTA236750.022.85817110
ATACGCA204000.021.567514
ATATACG205400.021.4273952
TATCAAC648200.021.3965452
GATATAC204500.021.2923471
CGCATTG202900.021.1829817
CCGAATT13300.021.08894714
GGGCTAC2350.021.06255510
TACGCAT211000.020.9301955
TATACGC211350.020.923013
ACGCATT215350.020.3414126
CGAATTA14100.020.28228615
AGTACGG261850.020.04747813
GAGTACG263350.019.985512
TAGCCGG1953.6379788E-1119.7425675
Produced by FastQC (version 0.11.5)