FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071515
Sequences flagged as poor quality0
Sequence length91
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA432961.4095975438830675No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA246890.8038052882697951No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT182410.59387631185262No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176210.5736908333509685No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155890.5075345554229753No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141180.4596428798166377No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT122590.39911900153507307No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA91800.2988753107180007No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT87080.2835083012780338No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA76760.24990924673980106No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA61720.2009431827616014No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60850.1981107043266922No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.18261346599316625No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50360.1639581769908335No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45490.1481028092000202No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA44060.14344712625528444No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA41360.13465667594004913No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.12469416558278244No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT36000.11720600420313755No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35810.11658741695873209No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.11632695917161401No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.11580604359737784No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.11443864021500791No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA34500.11232242069467349No Hit
GATATACGCATTGTGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33900.11036898729128784No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33580.1093271561428155No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC33240.10822021054756367No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC32320.1052249459957057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT79000.062.204251
AGCGTCA3000.060.9208343
TGGTATC82250.060.460062
ATATACG221900.050.2222
GATATAC215350.049.8431931
ATACGCA233150.047.798684
CGTCAGA3850.047.4723245
CGCATTG234650.047.1474347
TACGCAT236950.046.997785
TATACGC238100.046.751413
ACGCATT238800.046.4920466
GGTATCA189800.046.2053571
GCATTGT242200.045.098648
CATTGTG262150.041.536879
CAGTACG2300.038.813321
GTATCAA348900.037.429121
GCAGCGT5100.035.8416181
GACCTTA4550.034.5616767
CCCATTA901.3607132E-633.056749
CTCAGTT652.5533178E-432.6945424
Produced by FastQC (version 0.11.5)