FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869294
Sequences flagged as poor quality0
Sequence length61
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG656927.556937008652999No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66870.769244927492885No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34940.4019353636399193No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20240.23283262049433218No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAA18130.20856004987955742No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15520.17853568528023891No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13560.1559886528608273No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11830.1360874456743058No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9300.10698336811251428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT46400.046.3066061
TGGTATC47500.045.1106152
ACCTCTA257.8529946E-444.0027643
GTCCTAA403.927733E-641.2668341
GCATTGG403.9401802E-641.2478458
ATTGCCA551.9283107E-839.99790610
GTGTCAA358.64517E-539.3017461
ACTAGTC358.662774E-539.2881847
AGTGGTC358.6657106E-539.2859236
AGTCCCT504.1228304E-738.50020233
CCGGTTA504.1228304E-738.50020222
TAAGCAT651.9790605E-938.0749325
GATATAC44400.037.239291
GTCATAT604.134381E-836.681631
GGCTAGG604.134381E-836.681631
CCTTATC458.82745E-636.6752972
GGGGCAT751.9826984E-1036.666866
GAGTCCG300.0019258636.6647529
ATATACG45250.036.4118842
CAGTGGT2450.035.9330251
Produced by FastQC (version 0.11.5)