FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546243
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA62881.1511360328644944No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62461.1434471471487966No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA61231.1209296961242523No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA54280.9936969444002028No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT49180.9003319035667275No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45620.8351594436908116No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.7943351219146059No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42980.7868293049064244No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.7350208606792215No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29530.540601893296573No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA26990.49410244158735217No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAA26720.48915958648440344No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.4593193871591947No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT24270.44430775314283205No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.36119455993028743No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAA19610.358997735440088No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT17480.3200041007390484No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.30645701638281864No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA16660.3049924667226857No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA16440.3009649551573201No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAA16410.30041574903477025No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAA15640.28631945855599067No Hit
GTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAA15430.28247501569814165No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14820.27130782453962793No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13580.24860730480756732No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.24201683133696908No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAA13220.24201683133696908No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAA12690.23231418983858834No Hit
CAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAA11440.20943060139901104No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10830.19826341024049735No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.19496817350519824No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAA10590.19386976126009853No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.18910997486466646No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGG10280.1881946313270834No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT9340.17098617282052128TruSeq Adapter, Index 12 (95% over 22bp)
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATCTGTCTCTTATACACATC9190.168240142207772No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.16494490547247287No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8340.15267930206885946No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA8230.15066554628617668No Hit
AGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAA8150.14920099662604372No Hit
ATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATC7890.14444121023061166No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7800.1427935918629621No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.1347385687322309No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA7310.1338232251946478No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA7280.13327401907209793No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7060.12924650750673233No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA6960.12741582043156616No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA6900.12631740818646647No Hit
CTGTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAA6830.12503592723385012No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.12393751498875044No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.12320524015868395No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG6690.12247296532861748No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.12247296532861748No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6560.12009307213090145No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.11588249185801923No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAA6300.11533328573546937No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAA6270.11478407961291953No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.11331952995278659No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGGCGT6050.11075656804755392No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6040.11057349934003732No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGG5870.1074613313122548No Hit
AGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAA5820.10654598777467171No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.10544757552957201No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.10526450682205538No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAA5570.10196927008675626No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.10178620137923965No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTCA150.001028159485.023081
GACCTTG150.001028159485.023081
CTTAACA357.741619E-872.876921
GTCGCCT357.741619E-872.876921
CTCATGT750.068.018461
CCTAAGG259.148271E-567.999792
ATCATGT950.067.123481
CGCCTTT455.828042E-966.110893
AGCGTCA800.063.749793
CATATAC950.062.648581
TCGCCTT501.3400495E-859.4998132
GATATAC22400.058.0737951
CTTCAAG302.2542186E-456.6664852
TGGTATC19250.056.0777442
ATATACG23700.055.769862
GCAGCGT1000.055.2651
TTCATGT850.055.014931
GTGGTAT20550.052.5446741
ATACGCA25450.051.9349984
TATACGC25700.051.2644233
Produced by FastQC (version 0.11.5)