FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546243
Sequences flagged as poor quality0
Sequence length61
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5645010.33422853931309No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20700.3789522245593994No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAA15880.29071310753638946No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.24476286194971836No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10860.19881261636304723No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10800.19771420411794752No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10260.18782849391205012No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9660.17684437146105306No Hit
ATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATCTGACGCTGCCG7580.1387660802975965No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.12466978981881689No Hit
GTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6710.12283910274365072No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATCTGTCTCTTATACACATCTGACGCTGCCG6670.12210682791358425No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.11405180478285305No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6040.11057349934003732No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.11002429321748745No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5840.10691212518970496No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAA150.00576225155.0475581
GTCCATA150.00576225155.0475581
AAGTACT150.00578317354.997164
GTCTAGT202.6142594E-454.99212614
CAAGTAC351.5709593E-647.1404233
CAGTGGT950.046.3558351
GTGGTAT25450.044.2327161
CTGCTGA257.81949E-444.0380441
CATTTGT257.81949E-444.0380441
GAATGCC257.8548456E-443.9977266
GCTGAAT257.8548456E-443.9977263
TGGTATC26700.042.2417532
TACTGTA300.001921767836.6782072
TGTTATC300.001921767836.6782072
AGGTAGA300.001924361936.66813323
GCTGCCC604.1429303E-836.66813355
TCAGAGG300.001924361936.66813322
CAAGCCT300.001924361936.66813322
ATATTCA458.838819E-636.6647764
TTACTGC300.001926092836.6614228
Produced by FastQC (version 0.11.5)