FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67636
Sequences flagged as poor quality0
Sequence length91
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT18842.7854988467679935No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT7031.0393873085339167No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT4840.7155952451357265TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGG4060.6002720444733575No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGGCGT2160.3193565556804069No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCGT2140.3163995505352179No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT2010.29717901709148975RNA PCR Primer, Index 12 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGG2000.2957005145188953No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.29126500680111184No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGGCGT1580.23360340646992728No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT1550.22916789875214386No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.22325388846176591No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.21733987817138803No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA1420.20994736530841562No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTT1400.20699036016322667No Hit
GGTTGGGATGGGATAGGACTAGCACCATTTTAAGTCCCTGTCACCTCTAC1310.1936838370098764No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.18481282157430953No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA1230.1818558164291206No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGGCGG1220.1803773138565261No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.17298480099355373No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCGTAGT1160.17150629842095927No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCGTAGT1080.15967827784020344No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.15672127269501449No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACGCGTAGTAAT1050.15524277012242002No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGGCGG990.14637175468685315No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAA980.14489325211425869No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTTTGCCGT900.13306523153350286No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCGT880.1301082263883139No Hit
GGCTAGGGGCATCCCAGGGAGGGTTTTTGTATGAGCCTGTGTCACAGTGT880.1301082263883139No Hit
GTTCTGCTGAATGCCGCGATGGGTCAGGTAGAGGTGACAGGGACTTAAAA880.1301082263883139No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGGCGT860.12715122124312497No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTGT850.12567271867053048Illumina PCR Primer Index 12 (95% over 22bp)
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAA840.124194216097936No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGGCGTATGCCGT820.12123721095274706No Hit
CTGCTGAATGCCGCGATGGGTCAGGTAGAGGTGACAGGGACTTAAAATGG810.11975870838015258No Hit
ATTCAAATCGATCTGCGCCAGGCTCAGCGGCACGAGGCAGAGTCCCCAGG790.11680170323496364No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTGTGGCGG760.1123661955171802No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTGGT750.11088769294458573No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAA720.1064521852268023No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGGCGTGTGGCGG700.10349518008161333No Hit
GTTATATTCAAATCGATCTGCGCCAGGCTCAGCGGCACGAGGCAGAGTCC700.10349518008161333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT150.001025344884.999991
TGGTATC402.2555469E-974.375012
CACCAGG302.6212747E-670.833332
CTATACA259.09039E-568.01
CATATGA259.09039E-568.01
TCAAGCT200.003210368663.7500042
GTCAAGC200.003210368663.7500041
AAGCTCA200.003210368663.7500044
CAAGCTC200.003210368663.7500043
TTCGGAC200.003210368663.7500041
CCCATCT356.5439326E-660.7142832
ACCAGGC356.5439326E-660.7142833
TGATGCG302.2400264E-456.6666645
GATGCGG302.2400264E-456.6666646
ATGCGGC302.2400264E-456.6666647
TAACACT401.4435673E-553.1250045
CCGCGAG250.00776473651.01
CGTCAGA751.09139364E-1051.01
AGCTCAG250.00776473651.05
TATGCTG250.00776473651.05
Produced by FastQC (version 0.11.5)