FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69008
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT2690.3898098771156967No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA2300.33329469047067006No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC2200.3188036169719453No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT2190.3173545096220728No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC1930.27967771852538836No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT1790.2593902156271737No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC1750.25359378622768375No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA1640.23765360537908647No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTCCGCAA1580.22895896127985163No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.22895896127985163No Hit
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGG1310.18983306283329468No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.17099466728495247No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC1180.17099466728495247No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT1160.1680964525852075No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA1140.16519823788546256No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAG1120.1623000231857176No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTG1080.1565035937862277No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT1060.15360537908648272No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.12897055413865058No Hit
CTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGA880.1275214467887781No Hit
GGAGAAGCAAGCAGGCGAATCGTAATGAGGCGTGCGCCGCCAATATGCAC860.12462323208903316No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTC860.12462323208903316No Hit
GCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTG830.12027591003941572No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG820.11882680268954324No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC810.11737769533967075No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA810.11737769533967075No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT710.10288662184094598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC552.7939677E-854.090912
TAAGGCT250.00776541551.05
ATGGCTC354.7952982E-448.5714268
GGTATCA1500.048.1666641
GTATCAA3050.044.5901641
TATCAAC3250.044.4615362
GTGGTAT701.8645915E-742.51
CAACGCA3850.037.5324675
ATCAACG3850.037.5324673
TCAACGC3850.037.5324674
AGTCGAG601.5800598E-435.4166647
AACGCAG4100.035.2439046
TGGCGAG250.002780532534.028-29
CATTACA751.4636418E-534.02
GCATTAC751.4636418E-534.01
AACTGCA500.00277094634.06
ATACGGG500.00277094634.01
GCGCAGA1157.205017E-933.260871
CGCAGAC1359.822543E-1031.4814832
CGCAGAG4750.031.315798
Produced by FastQC (version 0.11.5)