FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70415
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT5860.8322090463679614No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.42320528296527726No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.3919619399275723No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.3919619399275723No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.2726691756017894No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.2527870482141589No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.24994674430164027No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.22154370517645386No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.17751899453241496No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.16757793083859973No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.15479656323226584No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.15337641127600654No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.14627565149470992No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.14201519562593196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC810.1150323084570049No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.11361215650074559No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.11077185258822693No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.10935170063196761No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.10225094085067102No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.10225094085067102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG501.70985E-1068.01
TGGTATC1400.063.7499962
AAGTACG1150.062.826091
GTGGTAT1400.057.678571
ATATCAA1300.055.576921
GTCGAGG1750.048.5714268
TATCAAC8650.045.2023122
GTATCAA7150.045.1748241
AGTCGAG2350.043.4042557
GGTATCA3050.041.8032761
TCGAGGT1950.041.4102559
ATCAACG9750.040.1025623
TCAACGC9800.039.897964
CAACGCA10000.039.1000025
AGTATCA559.4707066E-538.636361
AACGCAG10500.037.2380946
CGCAGAG12950.030.193058
AGAGTCG6950.029.9640275
ACGCAGA13150.029.7338437
GCAGAGT13200.029.6212129
Produced by FastQC (version 0.11.5)