FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70415
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11111.5777888234041044No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.46865014556557555No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.3379961655897181No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.20308172974508273No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.16331747496982177No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.13917489171341332No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1079315486757083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAC150.00576263954.99644552
AGAATAG150.00576263954.9964455
TGGTATC2450.046.0501442
GTGGTAT2400.045.960941
CAGTGGT358.445317E-539.39511
GGGAACC350.004075068531.4265447
CGGGAAC350.004075068531.4265446
ATTGCCC350.004075068531.42654432
GGTATCA4300.027.4982223
GTATCAA8350.026.420661
AGAGTAC5150.026.1633611
TATCAAC9100.025.0986252
CAGAGTA5600.024.55198910
CAACGCA10250.022.535135
ATCAACG10300.022.1587643
ACGGGGA2000.021.9985816
AACGCAG10750.021.7427836
TCAACGC10600.021.5316284
CGGGGAA1802.5465852E-1019.85982917
GAGTACG6900.018.73067512
Produced by FastQC (version 0.11.5)