FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55470
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12162.192175950964485No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.7120966288083649No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.38038579412294937No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.3028664142779881No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.21813592933117No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.1838831800973499No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.17486929872002885No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.12619433928249504No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12078601045610239No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11537768162970974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCTT150.0057569754.9945878
CCCACCC257.795534E-443.9956755
GTGGTAT1950.039.554631
CAGTGGT358.4755615E-539.3528181
TGGTATC2000.038.5657652
GACAGCC300.001893914436.7292981
TACGGAA350.004068335531.4254784
AGTGGTA453.7121773E-430.6077482
GTATCAA8250.029.0495361
CGGGAAA2400.026.35157417
AGAGTAC5950.026.34194411
TATCAAC9350.026.2211842
CAGAGTA6000.026.1224310
ACGGGAA2750.025.99744416
GTCGACA651.0714955E-425.3821188
TCGACAT550.001212584724.9975419
ATCAACG10000.024.2413373
TCAACGC9950.024.042864
CAACGCA10000.023.9226475
TACGGGA3050.023.44031715
Produced by FastQC (version 0.11.5)