FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53061
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19113.6015152371798496No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.8085034205913949No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.4786943329375624No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.33734758108591995No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.24311641318482502No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.22992404967867175No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15453911535779574No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15453911535779574No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.13569288177757677No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.11873127155537966No Hit
GTGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.11684664819735775No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.11496202483933587No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.10176966133318256No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGGC150.005723246455.07277740
GTGGAAT150.005723246455.07277743
GTGGTAT1500.044.0999071
AACTCAG257.846792E-443.9336476
TGGTATC1600.041.2266542
GAGGGTT504.0441955E-738.47820711
CAACTCA300.00192333336.611375
CGAGGGT558.5313513E-734.9801910
TCGAGGG658.1798134E-833.8269969
AGGGTTT601.684115E-632.0651712
GGGTGTG350.004038471731.4701655
GTATCAA7900.031.0513571
TATCAAC9300.027.7799662
GCACAAA400.00782828427.48443623
ACTCAGA400.00786445327.4585327
ATCAACT400.00786445327.4585323
GTCGAGG4400.026.2104158
GGTATCA3200.025.839791
ATCAACG9850.025.3677793
AGAGTAC6250.025.06580411
Produced by FastQC (version 0.11.5)