FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16215
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT6564.04563675609004No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.6598828245451742No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC1010.6228800493370337No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.5118717237126117No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT810.49953746530989823Illumina PCR Primer Index 10 (95% over 21bp)
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.4933703361085414No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.43786617329633054No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.43786617329633054No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.40703052728954675No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG580.3576934936786926No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT500.3083564600678384Illumina PCR Primer Index 10 (95% over 23bp)
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.2713536848596978No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.26518655565834104No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.2590194264569843No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.2590194264569843No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.2466851680542707No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.24051803885291398No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT310.1911810052420598No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1788467468393463No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1788467468393463No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1726796176379895No Hit
GTGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA260.16034535923527599No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA240.14801110083256244No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA230.14184397163120568No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.14184397163120568No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1356768424298489No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATATCGTATGCCGT220.1356768424298489No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.12950971322849214No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.12950971322849214No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11717545482577861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG258.410625E-785.2569661
ATATCAA202.8951556E-585.2569661
TGGTATC356.2091876E-660.8978352
GTGGTAT401.3699573E-553.2856031
GGTATCA950.049.3592951
GTCGAGG659.771793E-845.7659078
AGTCGAG853.3651304E-1044.996737
GTATCAA1950.043.721521
TATCAAC2450.041.7585142
ATCAACG2850.035.7868773
TCGAGGT601.5279191E-435.4140939
TCAACGC2900.035.1698574
CAACGCA3050.033.4401935
AACGCAG3200.031.8726866
CAGAGTC2250.028.3312784
AGAGTCG2250.028.3312785
CGGGGGA600.00661465628.3312746
ACGCAGA4000.025.4981487
CGCAGAG4000.025.4981488
GCAGAGT4200.024.2839499
Produced by FastQC (version 0.11.5)