FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16215
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12557.739747147702744No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.826395312981807No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.6537156953438175No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.44403330249768735No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.3515263644773358No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.2713536848596978No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.23435090965155722No Hit
GTGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1726796176379895No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.16034535923527599No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.14184397163120568No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11717545482577861No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11717545482577861No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.10484119642306505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC800.051.7134252
GTGGTAT800.051.7134251
GGTTTAA257.634951E-443.9925945
GTTTAAA300.00187174336.66049646
GGGGGAA652.9606272E-629.610418
GTATCAA3700.029.0713311
CGGGGGA705.272641E-627.4953717
AGAGTAC2950.027.02934611
TATCAAC4100.026.9077992
GGTATCA1650.026.744723
CAGAGTA3000.026.5788610
GTCGAGG1351.2914825E-1024.440338
CAACGCA4350.024.0189445
CGGGAAA1151.636181E-823.90901817
AACGCAG4500.023.829326
ATCAACG4450.023.5518843
TCAACGC4450.023.4791934
TACGGGA1202.5713234E-822.9128115
CGAGGTT1202.5713234E-822.9128110
ACGGGAA1202.5713234E-822.9128116
Produced by FastQC (version 0.11.5)