FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353675
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72662.054428500742207No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.3064960769067647No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7440.2103626210503994No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4540.1283664381140878No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.10602954690040291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7450.043.946861
TGGTATC7700.042.8591272
CACGACA300.001924721436.66317723
ACGGGAT350.004094899631.43002916
GTATCAA32950.028.8071861
GTACTGG606.285396E-527.512951
TTACGTT400.00785867527.50516546
CGACAAA400.00786953627.49738325
CGGGCAA400.00788040827.4896076
TATCAAC37400.025.4423542
GCTCCGC651.0865333E-425.38579253
AGAGTAC22450.025.23145111
CCATACT550.001223533425.0046964
CAGAGTA23650.024.30001310
AGCTCCG701.7966254E-423.57252152
GGTATCA15650.023.3816151
ATCAACG41000.022.9433333
AGTCCAC600.00203221222.91772719
ACGGGGC600.00203221222.91772716
TCAACGC41600.022.612424
Produced by FastQC (version 0.11.5)