FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221623
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8960.4042901684391963No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.3916561006754714No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.27253489033177963No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.2373399872756889No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.22786443645289525No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.18184033245646886No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.1696574813985913No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.15386489669393522No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.15296246328224056No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.13581622846004252No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.11054809293259274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG1500.070.849131
TGGTATC4100.062.1949582
GTGGTAT3850.061.831971
CCTAGAC250.007788082650.9998633
ATATCAA3500.042.509481
ATACGGG603.1921918E-642.509481
GTATCAA15300.036.6748471
GTCGAGG3400.036.24998
TATCAAC19300.035.8936842
GGTATCA7850.034.108193
CCGGGAC500.002784191433.999913
GTACACC751.4783325E-533.999914
AGTCGAG4200.033.3927657
TCGAGGT3600.033.0554669
ATCAACG21250.032.5999153
CAACGCA21350.032.4472245
TCAACGC21550.032.1460884
AACGCAG23100.030.3570636
TAAGGCT300.006801229428.33325878-79
GTTGGGC600.006794000528.3332583
Produced by FastQC (version 0.11.5)