FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561726
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.29783203910803485No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT12820.22822514891601958No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.2100668297354938No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8390.14936107639667737No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.14829293997429352No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.14188412143999032No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.12319173404827266No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5660.10076086917821143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8550.058.163622
GTGGTAT8650.052.5867921
ATACGGG1400.048.5848581
GTATCAA29050.037.6093641
TATCAAC36850.035.8719182
AAGTACG3900.034.881441
GGTATCA14200.034.4224053
ATCAACG40450.032.2590453
ATATCAA7200.031.8838181
TCGAGGT6400.031.8724639
CAACGCA41300.031.5894975
TCAACGC41300.031.5894974
GTATGGG703.9343166E-430.3655381
GTCGAGG6900.030.1787578
AACGCAG44350.029.7045126
CTCAACG600.00680530928.331087
CACGTCG300.00680816428.3310868-69
AGTCGAG8950.027.0648847
CGCAGAG50100.026.1256648
ACGCAGA51300.025.6802167
Produced by FastQC (version 0.11.5)