FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561726
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28210.5022021412574814No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8680.15452373577153275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA150.00578459754.99394228
GTGGTAT10300.040.8777771
TGGTATC10850.039.0349122
GGCCGAA300.001924984536.66589411
CGTACAG300.001924984536.66589424
CGGGGAC903.8198777E-1133.610417
AACGTCG350.00409828631.4279126
TCGTACA350.00409828631.4279123
ACCTAGC453.7935644E-430.554918
TACTAGG400.00786714827.5018712
GTAGCAC507.0246164E-427.501873
ACTTTCG400.00787399127.49697320
GTTGACC550.001225923724.99947226
TCGACGG550.001225923724.99947215
AGCCCCG1151.906119E-823.9125410
CGAGGGT2000.023.37450810
GGTATCA19400.022.8237193
GCGTGTG852.5677129E-522.6465829
GTATCAA35100.022.0309051
TCGAGGG2300.021.5212869
Produced by FastQC (version 0.11.5)