FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379664
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86040.6236301012420415No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23850.1728681765995199No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22700.1645328137865451No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC17500.12684247758874626No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT17150.12430562803697132No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT16230.11763733778659151No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT16160.11712996787623653No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT13950.10111157499217202No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT13890.10067668649758202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26400.044.0957871
TGGTATC27550.042.2336732
ATCGTAT358.668961E-539.28545422
CAATGCG651.9808795E-938.0739119
TCGTATC401.9022211E-434.37352823
GTACGCG658.401548E-833.84347514
AAGTTCG501.8207915E-532.9985923
TCGTCTA1300.031.73286254
TCGGGCG350.00410150431.42608511
CGTAGTC453.7976974E-430.55313916
ACGGGCT1152.1827873E-1128.69338216
CGTCTAC1500.027.50181255
CCGGACG400.00787670927.49782631
GTCGTAA400.00787670927.49782612
ACGTCAC400.00787670927.49782612
CGTAAGA400.00787670927.49782614
TTCGCCG400.00787670927.49782617
GCTCGTC1600.025.7829539
GTATCAA96150.025.645981
ATACGGG651.0883619E-425.387213
Produced by FastQC (version 0.11.5)