FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936354
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT42430.4531405857186492No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31850.34014913163184013No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23030.2459539874876382No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.2230993833528772No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19240.205477842781683No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.17237070595095444No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14620.15613752918233917No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.14983649346294242No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.1324285473229142No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10180.10871956546348924No Hit
ATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATACTCATC10120.10807878216999127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG3750.063.4669461
TGGTATC16400.058.826482
GTGGTAT16150.055.7897151
GCGTCGG559.563238E-538.634479
GGTATCA32200.036.032771
AGTATCA5200.035.9616971
GGCGTCG601.5957639E-435.4149328
CGACCCG250.002790102533.99833384-85
AGACGTA500.002789393833.9983339
ATACGGG4400.033.8069651
ATATCAA14400.033.0557021
GTATCAA56850.031.2490391
TATCAAC73100.030.639672
GGGCGTC755.904674E-428.3334587
AGTCGAG19350.028.1138177
ATCAACG79850.027.8899153
GTCGAGG15600.027.7870988
CAACGCA81850.027.1565025
TCAACGC82450.027.0619744
TCGAGGT15400.026.7681679
Produced by FastQC (version 0.11.5)