FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97102
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11481.1822619513501267No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.22141665465180943No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.14829766637144445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1600.042.9844631
TGGTATC1750.040.8720862
CGAGGGT606.2656596E-527.49587616
GCTGAGT400.00786374627.4817098
CCAGACA400.00786374627.4817093
ATAAGCC400.00786374627.4817093
TCGAGGG651.0838283E-425.36773315
GGTATCA3750.024.9424511
GTATCAA6850.024.8996121
GAGGGTT801.5904558E-524.0588917
AGGGTTT701.7859452E-423.56789418
CGAGGTT4400.023.10962110
TCGAGGT4450.022.849969
TATCAAC7750.022.7179832
GTCGAGG4950.021.6522588
GAGGTTT4850.021.53206311
CCTTAGC650.003211850321.1615812
AGGTTTT5050.020.67930612
AGTCGAG5750.020.0735977
CAACGCA9100.019.0160035
Produced by FastQC (version 0.11.5)