FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62365
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT11101.7798444640423314No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.41529704160987735No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.4136935781287581No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.40086587027980436No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.3335204040727972No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.2918303535636976No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.27900264571474387No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.22608835083780965No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.20524332558325983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1280.20524332558325983No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1090.1747775194419947TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.17157059247975628No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.1667602020363986No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.15232903070632567No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.14912210374408721No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.14270824981961036No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT840.13469093241401428RNA PCR Primer, Index 11 (95% over 23bp)
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.12988054197065663No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1266736150084182No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.1202597610839413No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10743205323498757No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA670.10743205323498757No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1010181993105107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG600.077.9166641
CGACTCT200.00320919863.756
TCGACTC200.00320919863.755
ATACGGG454.3495675E-756.6666681
TGGTATC1100.054.0909042
GTGGTAT950.053.684211
ATATCAA1350.053.5185171
CTACTGG250.00776192151.04
CACTGCG250.00776192151.09
GGTGGCC250.00776192151.03
ATATTAC250.00776192151.01
CAGTGCT354.79171E-448.571439
TATCAAC6950.043.4172672
CCCCAAC209.287425E-442.578-79
GTATCAA5650.041.371681
TCGAGGT1550.038.3870969
GTCGAGG1600.037.18758
GGTATCA2700.036.20371
ATCAACG8350.036.1377263
TCAACGC8550.035.2923974
Produced by FastQC (version 0.11.5)