FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62774
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13442.1410137955204385No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.5177302704941537No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.33771943798387866No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.199127027113136No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.17523178385955968No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.1608946379074139No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.13540637843693248No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.10832510274954599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT150.005760235754.99522454
AGGGGAT150.005760235754.99522442
TGGGCCA150.005760235754.99522424
TAGGAAG150.005760235754.99522433
GAAGGAT150.005760235754.99522411
GTGGTAT1700.048.6026461
TGGTATC1800.045.9024962
GTCAGAT350.004072164731.42583825
TACGGGC453.757094E-430.55289615
CTTACAA400.00782080127.4976110
GTATCAA7400.026.7971341
GGTATCA3550.025.6019571
AGAGTAC6350.022.95076211
CAGAGTA6650.022.74238410
TCGAGGT4100.022.1322239
TATCAAC9150.021.9730012
CGAGGTT4150.021.8655710
AGGTTTT4250.021.35108612
GTCGAGG4500.020.7759728
GAGGTTT4500.020.77597211
Produced by FastQC (version 0.11.5)