EGAF00005411934 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005411934
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29658
Sequences flagged as poor quality	0
Sequence length	91
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	2070	6.979567064535708	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	308	1.038505630858453	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	209	0.7047002495110931	RNA PCR Primer, Index 11 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	177	0.5968035605907344	No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	165	0.5563423022455999	No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	162	0.5462269876593162	No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.49227864319913683	No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.47541978555533077	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	109	0.367523096634972	No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92	0.31020298064603147	No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.28660057994470295	No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84	0.2832288084159417	No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78	0.26299817924337443	No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67	0.22590869242700118	No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT	62	0.20904983478319508	No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.20230629172567266	No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.18544743408186662	No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.18544743408186662	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	54	0.1820756625531054	No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.16858857643806055	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.15172971879425448	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT	45	0.15172971879425448	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGT	41	0.13824263267920966	No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.13149908962168722	No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.12812731809292602	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT	37	0.12475554656416481	No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.1213837750354036	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC	34	0.11464023197788119	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGCTCTCGTATGCCGT	33	0.11126846044911996	No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT	33	0.11126846044911996	No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA	32	0.10789668892035875	No Hit
TATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.10789668892035875	No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.10452491739159754	No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGTACTA	31	0.10452491739159754	No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC	31	0.10452491739159754	No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.10452491739159754	No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA	31	0.10452491739159754	No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC	31	0.10452491739159754	No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA	30	0.10115314586283633	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGG	25	8.743973E-7	85.0	1
TGGTATC	70	0.0	66.78571	2
GTGGTAT	65	0.0	65.38461	1
AGATGTG	20	0.0031925854	63.750004	5
AGTATCA	35	6.4538217E-6	60.714283	1
GTCGAGG	50	1.2829332E-8	59.5	8
ATATCAA	80	0.0	58.437504	1
AAGTACG	45	4.2715328E-7	56.666668	1
AGTCGAG	65	1.6989361E-9	52.30769	7
ATGTGTA	25	0.007721996	51.0	7
TGTGTAT	25	0.007721996	51.0	8
TCGAGGT	60	5.40349E-8	49.583336	9
GGTATCA	160	0.0	39.843754	1
TATCAAC	465	0.0	38.387096	2
GTATCAA	350	0.0	37.642857	1
ATCAACG	565	0.0	31.59292	3
CAACGCA	565	0.0	31.59292	5
TCAACGC	575	0.0	31.043478	4
CAGAGTC	295	0.0	30.254236	4
AGAGTCG	295	0.0	30.254236	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content