FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64787
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28734.434531618997639No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.37507524657724545No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.3071603871146989No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.15280843379072961No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.14663435565777086No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.12348156265917547No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGCC150.005761322654.9945957
GTGGTAT1800.042.7735751
TGGTATC1800.041.2459452
GTAGGTG400.0078230527.49729734
TAGTTTA400.0078230527.49729721
CAGTGGT400.0078230527.4972971
TGTAGGT400.0078230527.49729733
TACAGTG506.964028E-427.4972957
GTATCAA7300.026.3672711
TATCAAC7450.026.2054772
ATCAACG8200.023.4733033
TCAACGC8350.023.3809364
CAACGCA8450.022.7788285
AGAGTAC4750.022.57672911
CGAGGTT3600.022.150610
TCGAGGT3600.022.15069
ACAGTGC752.8342145E-421.9978398
AACGCAG8700.021.80826
CAGAGTA4800.021.76869410
GGTATCA3500.021.21221
Produced by FastQC (version 0.11.5)