FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288749
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11770.40762045929163393No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT8430.29194906302705814No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.2808667735645838No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.23203543562055626No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.21818257379246334No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.2005201749616449No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.18458938385933804No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.174892380579673No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.14995722928910576No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.13748965364382212No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.11324714544465955No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.10112589134507825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6200.060.324182
GTGGTAT5650.060.1785851
ATACGGG1300.058.847711
TAGCGCT354.8244366E-448.56437
GTATTGT452.9207551E-547.2234731
AAGTACG1550.046.614141
TTCGCTA209.3183044E-442.49744410-11
ATATCAA3950.037.6592251
GGTATCA11150.035.449373
GTATCAA19700.034.9501651
TATCAAC24550.034.2778932
GCTAGAG500.002785182334.00093
GTCTTAC500.002785182334.00091
GTCGAGG6250.032.6352128
TCGAGGT6050.031.6069349
ATCAACG27150.030.9952983
TCAACGC27700.030.3798684
CAACGCA28000.030.0543675
TCTGTAC1452.1700544E-929.3111233
AGTCGAG8000.029.2144647
Produced by FastQC (version 0.11.5)