FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913080
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.3219871205151794No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.27675559644280895No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT21330.23360494151662506No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19120.20940114776361327No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.17796907171332194No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.16898847855609586No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.15026065624041704No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12510.13700880536207122No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11990.13131379506724492No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10320.11302405046655277No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.1101765453191396No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.10108643273316686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG2400.063.757271
GTCGCAT200.00322321363.7502868
GTGGTAT17850.057.3874971
TGGTATC19800.055.811392
ATATCAA12350.041.3002551
AAGTACG5850.039.961821
GGTATCA31900.036.5068633
GTATCAA56350.035.4521371
TATCAAC71550.034.9286962
ATCAACG79400.031.5824763
TCAACGC81050.030.8870874
CAACGCA81000.030.7487345
AGCGTCA1551.3460522E-1030.1630783
GGATCCG1003.0946976E-629.7485079
AGTATCA5650.028.5873321
AACGCAG87400.028.4971146
GTCGAGG14200.028.1339288
TCGAGGT13800.026.7921319
GCGTCAG1755.529728E-1026.715874
CGCAGAG100350.024.8182498
Produced by FastQC (version 0.11.5)