FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206151
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT36331.7623004496703873No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA17580.8527729673879827No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.7771002808620866No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.6839646666763682No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.4438494113538135No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.36235574894131No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA6700.32500448700224593No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA6560.31821334846787064No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.30802664066630775No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.3075415593424237No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.3041459900752361No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA6190.3002653394841645No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5770.2798919238810386No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA5470.2653394841645202No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.2546676950390733No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC4660.22604789692992033No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT4620.2241075716343845Illumina PCR Primer Index 10 (95% over 22bp)
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.19791318014465123No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.18045025248482907No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.1629873248250069No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.15619618629063162No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.1557111049667477No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.13145703877255022No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.13000179480089838No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.12563606288594284No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT2510.12175541229487125Illumina PCR Primer Index 10 (95% over 24bp)
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.1115687044933083No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.10574772860670091No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.10235215933951326No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG2100.10186707801562933No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10041183404397747No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)