FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206151
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78613.8132242870517246No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17640.8556834553312863No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6440.31239237258126323No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5810.28183224917657446No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.19063696028639202No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.18481598439978464No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.13776309598304157No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.12418081891429097No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.11399411111272806No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.1067178912544688No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTAT150.00577286455.00971235
GATCAGG150.005778413354.9963644
GGATAGT150.005778413354.9963645
GTCAGTT150.005778413354.9963610
TTGACAC150.005778413354.9963625
GCGAGTT150.005778413354.9963633
ACGGGTG654.1836756E-1142.3048916
TGCTTAA551.9163053E-839.99735319
TACAAGG358.6216176E-539.3021932
GAATCCC358.6463304E-539.28311549
AAGGGTA358.6463304E-539.2831155
GTAACTT358.6463304E-539.2831159
GTACAAG458.769042E-636.6909561
GTGTATA751.9463187E-1036.6909561
CCCCTGA300.00192258636.6642416
TGTATCG300.00192258636.664245
GTGGTAT10750.036.3496441
TGGTATC11100.035.690642
GGTAACT401.8968858E-434.3727238
GAACTCG401.8968858E-434.37272320
Produced by FastQC (version 0.11.5)