FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617185
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74771.2114681983521958No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14790.23963641371711886No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10370.16802093375568103No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6550.10612701216004924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT150.005785110454.99335531
GTGGTAT12350.045.23731
TGGTATC12650.043.4765322
TGACGGT300.001926841236.6592676
TTAAACG951.8189894E-1234.73264322
TACATCG401.8991614E-434.37920841
CACGCAT453.7908083E-430.55929454
GTATCAA44900.025.8662621
CCGGGCT550.001225615325.00103242
CGGCCCG550.001226774324.99697928
GGTATCA23600.023.8867633
ACATCGA600.002033978722.91761442
ACGGGCT600.002034937722.9157563
TATCAAC51200.022.7725322
AGAGTAC32350.022.69616111
CAGAGTA33150.022.06548710
TATACTG1406.4665073E-921.602785
CGGGGAT1155.037127E-721.52088417
TCGAGGG2900.020.8612389
ATCAACG56400.020.3316383
Produced by FastQC (version 0.11.5)