FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378861
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT32860.8673365693486529No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.3539556723970005No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.30406930246185276No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.23966573492652976No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.21142318686800698No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6970.18397248595131196No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.1831806388094842No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.1488672626636154No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.14860331361633952No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5530.14596382314358036No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC4690.1237921031724036No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.11349809032864297No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.11006675271405608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG1400.066.785091
AAGTACG2150.065.231951
TGGTATC8550.052.6895642
GTGGTAT7900.052.183061
AGTATCA2250.041.5551641
ATATCAA5100.040.8329541
AGCGTCA450.001661715137.7774283
GTATCAA29200.037.114381
TATCAAC35950.036.1749082
GGTATCA14750.032.270893
ATCAACG40200.032.2447243
TCAACGC40500.032.110814
CAACGCA41800.031.1121525
GCGTCAG550.00444448530.90884
AACGCAG42800.030.0873366
GTCGAGG7600.029.074848
TCGAGGT7500.028.329339
AGTCGAG10300.027.2291647
CTAAAGT1001.0541579E-425.4997624
ACGCAGA51200.025.0648177
Produced by FastQC (version 0.11.5)