FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378861
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68551.8093707190763895No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11330.2990542705636104No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7610.20086522497697046No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.13250242173250876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA150.005779144455.00356718
TGGTATC7950.041.852532
GTGGTAT8200.040.63021
ACCGGGG350.00409489331.4306141
GTCCGAT350.00409754231.4264630
TATAGTG553.4926197E-529.9900635
GTATCAA35000.028.5572261
GTCTATA400.007818568527.5345131
TCGACGG400.00787423427.494529
ATAAACG651.0865973E-425.386263
GTATTAT550.00121610825.0313781
TATCAAC39700.024.935352
AGAGTAC25250.024.28228811
TTAAACG801.6037293E-524.06405822
GGTATCA15650.023.7518181
CAGAGTA26050.023.74766710
GCCCCGA600.002035382722.91209811
GCGTGCG600.002035382722.9120989
ATCAACG43050.022.8063563
TCAACGC43100.022.7708784
Produced by FastQC (version 0.11.5)