FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705939
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84230.3112782660658648No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32070.1185170840880005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT40950.039.7238961
TGGTATC41450.038.4936182
GGACGGC604.162939E-836.6585356
CGTTCGT1152.1827873E-1128.69618445
ACGTTCG1004.3201E-927.5005144
GCGTAAG400.007874276527.50050735
TCCGCGT507.034398E-427.553
CGAGTTA400.00787498727.549
CGCGTCA400.00787498727.555
TCCAACG400.00787640727.49898324
GTCCGTA550.001227116324.99953812
GTATCAA137800.024.6081941
CGGCTAG901.4487396E-624.44354222
GTTACGT1251.7480488E-924.19910429
AAGCGTC701.8010488E-423.57230234
TTGCGAT952.3175417E-623.15832343
ATCGCAA600.002036019222.91709145
TACATCG1950.022.5645241
GGTATCA75800.022.5496921
ACGGCGC1003.621768E-621.997568
Produced by FastQC (version 0.11.5)