FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378448
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59290.24928020288860633No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47210.19849078054260594No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33040.1389141154231667No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.13172455315398948No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.10557304595265485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29250.058.996432
GTGGTAT30100.054.9345051
ATACGGG5200.040.872271
CGACGTC450.001662371337.7825555
GTATCAA100350.036.5133741
ATGTGCG751.48456165E-533.9992948
GTCGAGG26400.033.96718
TATCAAC125700.033.5768172
TCGAGGT25950.033.0821659
GGTATCA55300.032.975721
AAGTACG11950.030.9466341
ATCAACG139600.030.2640153
CAACGCA141200.029.8922355
AGTCGAG34250.029.6575667
TCAACGC142850.029.5767154
GCGTCGG905.1581403E-528.3327459
AACGCAG151250.028.0746236
GGCGTCG957.4542615E-526.8415478
AGCGTCA3500.025.502153
AGACGGT850.001227089224.999488
Produced by FastQC (version 0.11.5)