FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378448
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61490.2585299321238051No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23950.10069591599227731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG257.861409E-443.9963317
CCGTCGG654.1836756E-1142.30327624
TGGTATC33500.040.8011132
GTGGTAT34400.040.1544951
GACCGTC651.9826984E-938.0729522
GGCGTCG1152.1827873E-1128.6938638
AGACCGT1103.965397E-1027.49886521
GCGTCGG1256.002665E-1126.401139
TCGGCAT1256.002665E-1126.40057612
GTCGGCA1256.002665E-1126.40057611
GTATCAA119850.024.9333521
GGTATCA65400.024.2344741
CAACGAG701.8024296E-423.56946419
GCCACGT600.00203416622.92054239
AGAGTAC78600.022.25239411
TATCAAC138400.021.6993622
ATACGGG1658.367351E-1121.666233
ACCATCG1406.4828782E-921.60488724
ACCGTCG1307.016206E-821.15163823
ATTACCC1701.2551027E-1021.0289863
Produced by FastQC (version 0.11.5)