FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853652
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT69740.816960541297859No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.7319141757999746No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42650.49961811136153844No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT37900.44397482815011263No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT30860.3615056252430733No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA28180.33011109913641623No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26650.3121881047546307No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.27329637838369736No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.24799332749176478No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19560.2291331830769447No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA18030.21121018869515915No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.18848430039407157No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA15730.18426712524541616No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.18332997521238162No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.16400125578104427No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA13570.15896407435348361No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.1581440680745784No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA13230.15498118671308683No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12870.1507640115644314No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA12220.1431496675460258No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.14303252379189646No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA11370.1331924484450338No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.13213815465786996No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTCCGCAA11060.12956099206702498No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC10760.12604667944314543No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC10660.12487524190185227No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA10250.12007234798255026No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.1197209167201623No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA9880.11573802907976552No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.11515231030911895No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA9810.1149180228008603No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA9790.11468373529260167No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACG9690.1135122977513085No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT8920.10449222868335108No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC8610.10086077230534221No Hit
TATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8580.10050934104295427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG3050.069.6901861
ACAGCGT259.139334E-568.0176241
ATATCAA14450.065.3110351
AAGTACG6000.060.2239381
CTATTAG302.252019E-456.681353
AGTATCA7300.052.4108431
TGGTATC37150.051.033112
CGTTGTG250.0077893951.010232
AGCGTCA605.6359568E-849.5961843
GCCTATT354.8201243E-448.5840151
GTGGTAT36400.048.4672281
GAGTTAG409.3061797E-442.5110171
GTATACT409.3061797E-442.5110176
TCCGATC1151.8189894E-1036.9661033
TTCCGAT1151.8189894E-1036.9639362
GGACCGT601.5927578E-435.4258466
AGGAGTC858.6828004E-735.007022
GTATCAA123200.034.3331641
GTCTCGC1008.043207E-834.0088121
CCTATTA500.002785845434.006822
Produced by FastQC (version 0.11.5)